pe conjugated anti cd86  (Elabscience Biotechnology)

Journal: Materials Today Bio

Article Title: Intravesical folate-conjugated hydroxyethyl starch micelles for pH-triggered co-delivery of epirubicin and TLR7 agonist toward synergistic chemoimmunotherapy of bladder cancer

doi: 10.1016/j.mtbio.2026.102835

Figure Lengend Snippet: (A) CLSM images of RAW 264.7 cells after treatment with LPS + IFN-γ and IL-4. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (B) Confocal imaging of CD86 and CD206 expression in M2-TAMs treated with free IMQ, FA-HES-EPI, and FA-HES-EPI/IMQ micelles. Cell nuclei were stained with DAPI (blue), CD86 fluorescence displayed in green and CD206 fluorescence displayed in red. Scale bar: 10 μm; (C) FCM analysis of expression of CD86 and CD206 after co-culturing M2-TAMs with different formulations; (D) Mean fluorescence intensity of CD86 and CD206 in M2-TAMs treated with different formulations. (n = 3), n.s.: p > 0.05, **p < 0.01and ****p < 0.0001 vs. the IL-4 group; ##p < 0.01and ####p < 0.0001 vs. the IL-4 group; (E) Expression levels of IL-6 and TNF-α in RAW264.7 cells treated with free IMQ and FA-HES-EPI/IMQ micelles. (n = 3), n.s.: p > 0.05, ***p < 0.001 and ****p < 0.0001; ####p < 0.0001; (F) Western blot analysis of TLR7/MyD88/NF-κB signaling in RAW 264.7 cells treated with free IMQ or FA-HES-EPI/IMQ micelles.

Article Snippet: Rabbit anti-CD86 polyclonal antibody, rabbit anti-CD206 polyclonal antibody, FITC goat anti-rabbit antibody, IgG/Alexa Fluor555 goat anti-rabbit antibody, APC anti-mouse CD206 antibody, PE anti-mouse CD86 antibody, purified anti-mouse CD16/32 antibody, intracellular Fixation/Permeabilization buffer kit, IL-6 ELISA kit and TNF-α ELISA kit were all purchased from Elabscience Biotechnology (Wuhan, China).

Techniques: Staining, Fluorescence, Imaging, Expressing, Western Blot

Journal: Emerging Microbes & Infections

Article Title: Abolishing PorB-induced mitophagy enhances gonococcal outer membrane vesicle vaccine efficacy

doi: 10.1080/22221751.2026.2651468

Figure Lengend Snippet: Impact of gonococcal OMVs on dendritic cell activation and T cell differentiation. Mature murine bone marrow-derived dendritic cells (BMDCs) were stimulated with OMV-PorB WT , OMV-PorB K117Q/K171Q or a Mock control and surface expression of CD86 and MHCII was determined by flow cytometry. Stimulated BMDCs were subsequently co-cultured with naïve murine T cells and IFN-γ, TNF-α, and IL-4 cytokine levels in the culture supernatant was determined by ELISA. (A) Representative flow cytometry repeat for detection of CD86 levels at the surface of OMV-stimulated DCs. (B) Quantification of CD86 levels at the surface of OMV-stimulated DCs. (C) Representative flow cytometry repeat for detection of MHCII levels at the surface of OMV-stimulated DCs. (D) Quantification of MHCII levels at the surface of OMV-stimulated DCs. (E) Quantification of IL-2 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (F) Quantification of IL-10 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (G) Quantification of IL-4 secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (H) Quantification of IFN-γ secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (I) Quantification of TNF-α secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. (J) Quantification of IL-17A secretion in the culture supernatant after co-culture of T cells with OMV-stimulated DCs. Graphs represent mean ± SD of three biological repeats. Significant differences were identified by one-way ANOVA (GraphPad Prism).

Article Snippet: Mature DCs (1 × 106 cells/well) were stimulated for 20 h with 20 μg of OMV-PorB WT or OMV-PorB K117Q/K171Q or with a PBS-only Mock control. for 20 h. Expression of CD86 and MHCII was analyzed by flow cytometry on CD11c-positive cells (FITC-labeled anti-mouse CD11c; Elabscience, #E-AB-F0991C) using PE-labeled anti-mouse CD86 (Elabscience, #E-AB-F0994D) and APC-labeled anti-mouse MHCII (Elabscience, #E-AB-F0990E).

Techniques: Activation Assay, Cell Differentiation, Derivative Assay, Control, Expressing, Flow Cytometry, Cell Culture, Enzyme-linked Immunosorbent Assay, Co-Culture Assay